Die chronologische Liste zeigt aktuelle Veröffentlichungen aus dem Forschungsbetrieb der Hochschule Weihenstephan-Triesdorf. Zuständig ist das Zentrum für Forschung und Wissenstransfer (ZFW).
Obtaining of hydroponically grown Brassica plants reach on glucosinolates (2008) International Symposium on Strategies Towards Sustainability of Protected Cultivation in Mild Winter Climate. Ege University, Turkey, Antalia .
Methods for obtaining of glucosinolates from plants, cell and transformed root cell cultures (2008) TU Berlin (Visit of General Mills, USA and Netherlands) .
H. Frank,
Markus Gandorfer,
Prof. Dr. Patrick Noack
Ökonomische Bewertung von Parallelfahrsystemen (2008) Tagungsband zur 28. GIL-Jahrestagung 10. – 11. März 2008 Christian-Albrechts-Universität zu Kiel , S. 47-50.
Prof. Dr. Iryna Smetanska
Plant system for production of biologically active substances (2008) HU Berlin, Charite .
Prof. Dr. Iryna Smetanska
Research directions in the Department of Methods in Food Biotechnology (2008) ProFil Mentoren program, TU Berlin .
Prof. Dr. Iryna Smetanska
Obtaining of steviosides from Stevia and its cell cultures (2008) TU Berlin (visit of Dr. Ozaki, Japanese Institute of Fruits and Vegetables) .
Prof. Dr. Lydia Nausch,
Jonathan Ledoux,
Adrian D. Bonev,
Mark T. Nelson,
Wolfgang R. Dostmann
Berechtigungen: Peer Reviewed
Differential patterning of cGMP in vascular smooth muscle cells revealed by single GFP-linked biosensors (2008) PNAS 105 (1), S. 365–370.
DOI: 10.1073/pnas.0710387105
Here, we report the design of unprecedented, non-FRET based cGMP-biosensors, named FlincGs, to assess the dynamics of nitric oxide (NO) and atrial natriuretic peptide (ANP) induced synthesis of intracellular cGMP, [cGMP](i). Regulatory fragments of PKG I alpha, PKG I beta, and an N-terminal deletion mutant of PKG I alpha were fused to circular permutated EGFP to generate alpha-, beta-, and delta-FlincG, with high dynamic ranges and apparent K(D,cGMP) values of 35 nM, 1.1 microM, and 170 nM, respectively. All indicators displayed significant selectivity for cGMP over cAMP, and 1.5- to 2.1-fold increases in fluorescence intensity at 510 nm when excited at 480 nm. Surprisingly, FlincGs displayed an additional excitation peak at 410 nm. delta-FlincG permitted ratiometric (480/410 nm) measurements, with a cGMP-specific 3.5-fold ratio change. In addition, delta-FlincG presented cGMP association and dissociation kinetics sufficiently fast to monitor rapid changes of [cGMP](i) in intact cells. In unpassaged, adenoviral transfected vascular smooth muscle (VSM) cells, delta-FlincG had an EC(50,cGMP) of 150 nM, and revealed transient global cGMP elevations to sustained physiological NO (EC(50,DEA/NO) = 4 nM), and the decay phase depended on the activity of PDE-5. In contrast, ANP elicited sustained submembrane elevations in [cGMP](i), which were converted to global cGMP elevations by inhibition of PDE-5 by sildenafil. These results indicate that FlincG is an innovative tool to elucidate the dynamics of a central biological signal, cGMP, and that NO and natriuretic peptides induce distinct cGMP patterning under the regulation of PDE-5, and therefore likely differentially engage cGMP targets.
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Gerhard Radlmayr
Referent für Wissenstransfer und Forschungskommunikation
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