Die chronologische Liste zeigt aktuelle Veröffentlichungen aus dem Forschungsbetrieb der Hochschule Weihenstephan-Triesdorf. Zuständig ist das Zentrum für Forschung und Wissenstransfer (ZFW).
Prof. Dr. Jörg Kleiber,
Thomas Walter,
Gerd Haberhausen,
Sue Tsang,
Reiner Babiel,
Maurice Rosenstraus
Berechtigungen: Peer Reviewed
Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA (2000) The Journal of Molecular Diagnostics 2 (3), S. 158-166.
DOI: 10.1016/S1525-1578(10)60632-0
We developed a homogeneous format reverse transcription-polymerase chain reaction assay for quantitating hepatitis C virus (HCV) RNA based on the TaqMan principle, in which signal is generated by cleaving a target-specific probe during amplification. The test uses two probes, one specific for HCV and one specific for an internal control, containing fluorophores with different emission spectra. Titers are calculated in international units (IU)/ml by comparing the HCV signal generated by test samples to that generated by a set of external standards. Endpoint titration experiments demonstrated that samples containing 28 IU/ml give positive results 95% of the time. Based on these data, the limit of detection was set conservatively at 40 IU/ml. All HCV genotypes were amplified with equal efficiency and accurately quantitated: when equal quantities of RNA were tested, each genotype produced virtually identical fluorescent signals. The test exhibited a linear range extending from 64 to 4,180,000 IU/ml and excellent reproducibility, with coefficients of variation ranging from 21.6 to 30.4%, which implies that titers that differ by a factor of twofold (0.3 log10) are statistically significant (P = 0.005). The test did not react with other organisms likely to co-infect patients with hepatitis C and exhibited a specificity of 99% when evaluated on a set of samples from HCV seronegative blood donors. In interferon-treated patients, the patterns of viral load changes revealed by the TaqMan HCV quantitative test distinguished responders from nonresponders and responder-relapsers. These data indicate that the TaqMan quantitative HCV test provides an attractive alternative for measuring HCV viral load and should prove useful for prognosis and for monitoring the efficacy of antiviral treatments.
D. Bucher,
M. Scholz,
Prof. Dr. Martin Stetter,
Klaus Obermayer,
Hans-Joachim Pflügler
Berechtigungen: Peer Reviewed
Correction methods for three-dimensional reconstructions from confocal images: I. tissue shrinking and axial scaling (2000) Journal of Neuroscience Methods 100 (1-2), S. 135-143.
DOI: 10.1016/S0165-0270(00)00245-4
Prof. Dr. Winfried Ruß,
Roland Meyer-Pittroff
Stoffliche und energetische Verwertung von Rückständen der Lebensmittelindustrie (2000) Tagungsband zu den EXPO 2000 Workshops: Biotechnologie und Altlastensanierung, 07.-12. Juni 2000 .
Werner Back,
Prof. Dr. Martin Krottenthaler,
Tobias Voigt,
Prof. Dr. Ulrich Hege,
B. van de Braak,
K. Stippler
New Control System for the Lautering Process in the Lauter Tun (2000) Brauwelt International 18 (6), S. 475-477.
Hauke Bartsch,
Prof. Dr. Martin Stetter,
Klaus Obermayer
Berechtigungen: Peer Reviewed
The influence of threshold variability on the response of visual cortical neurons (2000) Neurocomputing 32-33 , S. 37-43.
DOI: 10.1016/S0925-2312(00)00141-7
Holger Schöner,
Prof. Dr. Martin Stetter,
Ingo Schießl,
John E. W. Mayhew,
Jenny S. Lund,
Niall McLoughlin,
Klaus Obermayer
Berechtigungen: Peer Reviewed
Application of Blind Separation of Sources to Optical Recording of Brain Activity (2000) S. A. Solla, T. K. Leen und K.-R. Müller (Hrsg.), Neural Information Processing Systems (NIPS) 12, MIT Press, Cambridge, MA , S. 949-955.
Torsten Dickel,
Prof. Dr. Martin Krottenthaler,
Werner Back
Berechtigungen: Peer Reviewed
Untersuchungen zum Einfluß des Kühltrubeintrages auf die Bierqualität (2000) BrewingScience - Monatsschrift für Brauwissenschaft 53 (5/6), S. 95-99.
Prof. Dr. Martin Stetter,
I. Schießl,
T. Otto,
F. Sengpiel,
Mark Hübener,
Tobias Bonhoeffer,
Klaus Obermayer
Berechtigungen: Peer Reviewed
Principal Component Analysis and Blind Separation of Sources for Optical Imaging of Intrinsic Signals (2000) NeuroImage 11 (5), S. 482-490.
DOI: 10.1006/nimg.2000.0551
Betreuung der Publikationsseiten
Gerhard Radlmayr
Referent für Wissenstransfer und Forschungskommunikation
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