Die chronologische Liste zeigt aktuelle Veröffentlichungen aus dem Forschungsbetrieb der Hochschule Weihenstephan-Triesdorf. Zuständig ist das Zentrum für Forschung und Wissenstransfer (ZFW).
Dr. rer. nat. Heidi Riedel,
Christian Ulrichs,
Prof. Dr. Iryna Smetanska,
Inga Mewis
Characterization and accumulation of bioactive phenolic acids in plant cell cultures (2007) XXXVII Annual ESNA Meeting. Dubna, Russia , S. 67-68.
Prof. Dr. Iryna Smetanska
Comparison of different systems for glucosinolate recovering (2007) XXXVII Annual ESNA Meeting, Dubna, Russia (chairperson) .
Prof. Dr. Iryna Smetanska
Development on the methods in food biotechnology concerned the recovery of the valuable plant metabolites (2007) XXXVII Annual ESNA Meeting, Dubna, Russia (chairperson) .
Prof. Dr. Iryna Smetanska
Production of phenolics from medicinal plants by cell cultures (2007) XXXVII Annual ESNA Meeting. Dubna, Russia (chairperson) .
Prof. Dr. Iryna Smetanska
Obtaining of secondary metabolites from cell and root cultures (2007) Timirjasev Academy, Moscow, Russia .
Prof. Dr. Iryna Smetanska,
Monika Schreiner,
Ilona Schonhof,
Angelika Krumbein,
Prof. Dr. Dietrich Knorr,
Kersten Maikath
Receiving of glucosinolates from exudates of Capparales (2007) Patent 10 2006 004 829.6/42, PCT/EP 2007/000788 .
Prof. Dr. Lydia Nausch,
Wolfgang R. Dostmann
Berechtigungen: Open Access
Berechtigungen: Peer Reviewed
FlincGs: novel, non-FRET cGMP biosensors with nanomolar sensitivity for NO-induced signaling (2007) BMC Pharmacology 7 (S1), S. 1.
DOI: 10.1186/1471-2210-7-S1-S22
Here, we report the design of unprecedented, non-FRET based cGMP-biosensors, named FlincGs, to assess the dynamics of nitric oxide (NO) and atrial natriuretic peptide (ANP) induced synthesis of intracellular cGMP, [cGMP]i. Regulatory fragments of PKG I α, PKG I β, and an N-terminal deletion mutant of PKG I α were fused to circular permutated EGFP to generate α-, β-, and δ-FlincG, with high dynamic ranges and apparent KD,cGMP values of 35 nM, 1.1 μM, and 170 nM, respectively. All indicators displayed significant selectivity for cGMP over cAMP, and 1.5- to 2.1-fold increases in fluorescence intensity at 510 nm when excited at 480 nm. Surprisingly, FlincGs displayed an additional excitation peak at 410 nm. δ-FlincG permitted ratiometric (480/410 nm) measurements, with a cGMP-specific 3.5-fold ratio change. In addition, δ-FlincG presented cGMP association and dissociation kinetics sufficiently fast to monitor rapid changes of [cGMP]i in intact cells. In unpassaged, adenoviral transfected vascular smooth muscle (VSM) cells, δ-FlincG had an EC50,cGMP of 150 nM, and revealed transient global cGMP elevations to sustained physiological NO (EC50,DEA/NO = 4 nM), and the decay phase depended on the activity of PDE-5. In contrast, ANP elicited sustained submembrane elevations in [cGMP]i, which were converted to global cGMP elevations by inhibition of PDE-5 by sildenafil. These results indicate that FlincG is an innovative tool to elucidate the dynamics of a central biological signal, cGMP, and that NO and natriuretic peptides induce distinct cGMP patterning under the regulation of PDE-5, and therefore likely differentially engage cGMP targets.
Autor Unbekannter,
Prof. Dr. Iryna Smetanska
Pickled cucumbers: cultivation and processing (2007) ÖKO-TEST Magazin .
Betreuung der Publikationsseiten
Gerhard Radlmayr
Referent für Wissenstransfer und Forschungskommunikation
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